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- Ferric chloride method: Green color is positive for phenylpyrubic acid
- Phenastix method (urine reagent dip strip): Persistent blue-gray to green-gray color is positive for phenylpyrubic acid
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- To assist in the detection of hyperphenylalaninemia, including PKU (phenylketouria)
- To monitor the effect of dietary treatment for patients who have a defect in amino acid metabolism
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- PKU (phenylketouria)
- Non-PKU hyperphenylalaninemia
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- Inadequate labeling
- Improper collection procedure
- Diluted urine
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